Engineered regulatory states resolve context-dependent genetics in macular degeneration

Norman/Dey labs (MSKCC): CRISPRa Multiome Perturb-seq of 119 TFs + dual-guide Perturb-seq of 1,225 TF pairs in RPE-1; chromatin priming predicts emergent states; Mosaic (Transformer) predicts TF-pair interaction residuals (r=0.484) and nominated a validated triple-TF combination.Norman/Dey 实验室（MSKCC）：在 RPE-1 中对 119 个 TF 做 CRISPRa Multiome Perturb-seq、对 1,225 个 TF 对做双向导 Perturb-seq；染色质预激活可预测新兴状态；Mosaic（Transformer）预测 TF 对相互作用残差（r=0.484），并提名经实验验证的三 TF 组合。

Authors / organisation作者 / 机构

Metzner E, Guruvayurappan K, Southard KM, Ardy RC, Dey KK, Norman TM.

Venue发表于

bioRxiv

Date日期

2026-10-12

Type类型

preprint预印本

Year年份

2026

DOI

10.64898/2026.10.09.758047

Verification核验来源

Crossref;PDF

perturbationPerturb-seqMultiomeCRISPRaTF-combinationschromatin-primingMosaicgenetic-interactionAMDomnigenic

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