{"scope":{"layer":"general","tissue_scope":"neural","species":"mouse","system":"organoid","evidence_level":"preprint","importance":"","importance_reason":""},"id":"VC0156","type":"preprint","title":"A perturbation atlas of mouse neural organoids resolves macroglial reactivity and nominates drivers of degeneration","authors_or_org":"Dupré J-S, Signer M, Rao J, Adam L, Gaspa-Toneu L, Almato-Bellavista M, Cubela I, Sadiraj L, Fuhrer T, Scherer J, Parés i Borrell T, Schröter M, Hierlemann A, Zampieri M, Lutolf MP, Camp JG.","date":"2026-10-11","year":"2026","venue":"bioRxiv (preprint, not peer-reviewed)","url":"https://doi.org/10.64898/2026.10.09.757894","doi":"10.64898/2026.10.09.757894","pmid":"","brain_tumor_focus":"no","tags":["perturbation","organoid","scRNA-seq","atlas"],"tag_slugs":["perturbation","organoid","scrna-seq","atlas"],"tag_labels":["Perturbation","Organoid","scRNA-seq","Atlas"],"tag_labels_zh":["扰动","类器官","单细胞 RNA 测序","细胞图谱"],"unmapped_tags":[],"one_line_summary_en":"Preprint (not peer-reviewed): Dupré et al. (Camp lab, Roche Institute of Human Biology) map mouse neural organoids spanning multiple brain regions and the retina; benchmarked against in vivo references, they reach postnatal neuro-macroglial transcriptomic states within a month. A single-cell perturbation screen of 13 Müller-glia-specific pathways maps cytokine-driven degeneration-like stress programs in photoreceptors and reactive Müller-glia states (including an Oncostatin M-driven chaperone-associated state), and gp130–STAT3 signalling is nominated as a targetable driver. Mouse, non-tumour; small (13-pathway) screen.","one_line_summary_zh":"预印本（未经同行评审）：Dupré 等（罗氏人类生物学研究所 Camp 实验室）构建覆盖多个脑区与视网膜的小鼠神经类器官单细胞图谱；与体内参考比对，类器官在一个月内达到出生后神经–大胶质细胞转录组状态。针对 13 条 Müller 胶质特异通路的单细胞扰动筛选显示，部分炎症细胞因子驱动光感受器的类退行性应激程序及 Müller 胶质的特定反应性状态（含 Oncostatin M 驱动的伴侣蛋白相关状态）；gp130–STAT3 信号被提名为可干预的驱动因素。小鼠、非肿瘤；筛选规模小（13 条通路）。","verified":"yes","verification_source":"bioRxiv API details (title, 16 authors, posted 2026-10-11, v1, new results, CC BY-NC-ND, corresponding author J. Gray Camp, Roche Institute of Human Biology, abstract) + Crossref posted-content record (author spellings Dupré, Parés i Borrell, Lutolf MP) fetched 2026-10-20; full text not read","page_url":"https://gliostate.com/library/VC0156/","api_url":"https://gliostate.com/api/items/VC0156.json","sameAs":["https://doi.org/10.64898/2026.10.09.757894"],"is_preprint":true,"facets":{"type":"https://gliostate.com/library/type/preprint/","year":"https://gliostate.com/library/year/2026/","tags":["https://gliostate.com/library/tag/perturbation/","https://gliostate.com/library/tag/organoid/","https://gliostate.com/library/tag/scrna-seq/","https://gliostate.com/library/tag/atlas/"]},"cited_by":[],"related":[{"id":"VC0090","title":"A State-Resolved Single-Cell and Spatial Atlas Reveals IDH-Associated Differences in Malignant and Immune Architecture in Diffuse Glioma.","shared_tags":["atlas"],"page_url":"https://gliostate.com/library/VC0090/","api_url":"https://gliostate.com/api/items/VC0090.json"},{"id":"VC0091","title":"A single-cell atlas of RNA alternative splicing in the glioma-immune ecosystem.","shared_tags":["atlas"],"page_url":"https://gliostate.com/library/VC0091/","api_url":"https://gliostate.com/api/items/VC0091.json"},{"id":"VC0060","title":"Charting the single-cell and spatial landscape of IDH-wild-type glioblastoma with GBmap.","shared_tags":["atlas"],"page_url":"https://gliostate.com/library/VC0060/","api_url":"https://gliostate.com/api/items/VC0060.json"},{"id":"VC0069","title":"A single-cell atlas of glioblastoma evolution under therapy reveals cell-intrinsic and cell-extrinsic therapeutic targets.","shared_tags":["atlas"],"page_url":"https://gliostate.com/library/VC0069/","api_url":"https://gliostate.com/api/items/VC0069.json"},{"id":"VC0160","title":"Spatially restricted iron-metabolism-associated stress programs prime invasive mesenchymal glioblastoma states in human tissue-context models","shared_tags":["scrna-seq","organoid"],"page_url":"https://gliostate.com/library/VC0160/","api_url":"https://gliostate.com/api/items/VC0160.json"},{"id":"VC0148","title":"AI-Driven Precision Oncology Startup aiPTO Unveils #1-Ranked DELPHAI Model, Bridging the \"Scale Gap\" From Virtual Cells to Virtual Tumors","shared_tags":["perturbation","organoid"],"page_url":"https://gliostate.com/library/VC0148/","api_url":"https://gliostate.com/api/items/VC0148.json"}]}